AFM and STM probing of horseradish peroxidase

 

These two images represent the nanomechanical and electrochemical probing of an HRP enzyme (horseradish peroxidase).

	During catalysis of hydrogene peroxyde to water, where GOx (glucose oxidase) supplies the H2O2 in a chain catalysis process, the HRP enzyme is mechanically probed by an AFM tip. The stiffness and adhesion is an indicator for the catalysis. During probing an electron is released through the SAM where the enzymes are attached to, and flows through the gold sustrate.
	The second image is a concept of an electron hopping through the tip attached redox polymer: schematic illustration of the oxidation of ferrocene moieties in poly(ferrocenylsilanes) (PFS) under coupling to a redoxactive enzyme attached to a conductive substrate.